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KMID : 0357419940240020123
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Korean journal of Virology
1994 Volume.24 No. 2 p.123 ~ p.128
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Plaque Assay for Varicella-zoster Virus Titration
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우규진
황규계/전복환/박송용/문흥모
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Abstract
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Titration of viral infectivity is important not only for virological research but also for the verification of efficacy of live vaccine. Pladue assay has been a method for live varicella-zoster virus(VZV) titration. Because VZV is very unstable
in
vitro, it is required to develop more stable and sultable plaque assay method to get reliable plaque titer,
In this study, the most suitable conditions of VZV titration was investigated. LuMA cell which is one of the host cell lines for VZV propagation was plated on 6-well plate as 5.5×10E5/well and incubated for one day before virus infection. The
highest
absorption was obtained at 60-80 minutes after VZV inoculation. The size of plaques was adequate to count on five days postinoculation under microscope, because the plaques started to fuse on day six or seven. The virus was stable in diluent
buffer
containing 30% FBS.
Using this improved method, we could titrate the live VZV vaccine more accurately and conveniently.
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KEYWORD
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